Figure 1.

Mo-DC differentiation in RA and PsA patients. Monocytes from HC, PsA, or RA patients were differentiated for 7 days with GM-CSF/IL-4. (A) Gating strategy: Cells were gated, doublets and dead cells excluded. Cells were gated for CD11c expression. (B) Representative histograms and (C) dot-plot representation of at least 3 separate experiments (n HC = 8, PsA = 9, RA = 12) of CD209 (Top), Mo-DC surface differentiation marker and CD14 (bottom), monocyte markers were evaluated by flow cytometry in the CD11c⁺ population. Data are represented as mean ±SEM and differences among groups were evaluated by two-way ANOVA with Bonferroni post-test. *p < 0.05, **p < 0.01, ***p < 0.001. SS, side scatter; FS, forward scatter; Lin, linear scale.