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. 2020 Jul 7;11:1437. doi: 10.3389/fimmu.2020.01437

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Copyright © 2020 Wang, Wang, Huang, Xu, Chen, Dai and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Recombinant antigen production and evaluation of rBmSP44 as a diagnostic antigen. (A) SDS-PAGE analysis of bacterial lysate as stained by Coomassie blue. Lane 1: induced protein; Lane 2: non-induced control; Lane 3 and 5: The purification of rBmSP44 after cleavage of GST tag; (B) Western blot analysis of rBmSP44; M: Protein marker; Lane 1–4: The recombinant proteins; Protein incubated with an anti-rBmSP44 monoclonal antibody (~24 kDa); (C–E) rBmSP44 recognized by sera from B. microti infected mice [Sera from day7 (C), 14 (D), and 21 (E) p.i.] Significant differences are as follows: ***P < 0.001, compared to non-infected control sera, t-test. The data shown are representative of at least 3 independent experiments.