Supplementary Fig. 2.

(A) IC50 shift assay. Effects of melatonin (Mel) and shikonin (SHK) on (B) cell morphology 24 h post-treatment and (C) 48 h post-treatment in HeLa cells. (D) Use of NAC pre-treatment to demonstrate the effect on the reversal in the morphological changes induced by Mel-SHK in these cells. (E) Effects of NAC on cell cycle regulators, CDC25C and p-cdc2 in Mel-SHK treated HeLa cells. The data in each bar graph are presented as the means ± standard errors of the means (SEM). ^###P < 0.001 compared with Mel-SHK treated cells.