Fig. 1.

Sodium nitroprusside enhances generation of cardiac conduction system cells. A. Flow cytometry analysis of Cntn2-EGFP expression. Murine ESC-derived cardiomyocytes were treated for five days with either DMSO or sodium nitroprusside (SN) and harvested at Day 25 of differentiation. Quantification of Cntn2-EGFP expression is shown in the right panel. B. Comparison of action potential duration, quantified by APD(50) and APD(90) between GFP− (black bars) presumptive ventricular cardiomyocytes and GFP⁺ presumptive Purkinje-like myocytes (gray bars). Adapted from (16).