Skip to main content
. 2020 Jul 7;11:826. doi: 10.3389/fphar.2020.00826

Figure 2.

Figure 2

Effect of CAPE on OM induced calcific-related gene/protein expression in AVICs. (A) AVICs were stimulated with OM and then treated or not treated with 10 μM CAPE for 24 h, 48 h and 7 days, the mRNA expression levels of RUNX2, ALP were detected by qRT-PCR. (B) The immunofluorescent staining of ALP, RUNX2 on AVICs with above conditioned culturing for 48 h, (C, D) The protein expression levels of the above genes were determined by Western blot and quantification analysis for 48 h (C) and 7 days (D) treatment with CAPE. *p<0.05 compared with control, #p< 0.05 compared with OM.