FIGURE 1.

Healthy brain tissue is largely devoid of fibrillar collagen. Second Harmonic Generation (SHG) imaging of mouse brain tissue shows virtually no fibrillar collagen (green, 890 nm excitation 445/40 nm emission) within the parenchyma. Collagen fibers can be seen surrounding the cortex and within and between ventricles. Multiphoton induced autofluorescence (red, 890 nm excitation, 592/100 nm emission, likely FAD) was recorded to observe gross anatomical features of the tissue. Scale bar = 1 mm.