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Immunohistochemistry of Collagen I overlaid with GFAP of the astrocytic glial scar in chronic horizontal slices. We re-registered the two files with fine-structures registration (BUNWARPJ, FIJI). We used a mask from SHG to see if all the pixels are co-registered. We found 88% of the SHG pixels belong to the collagen 1 antibody staining. Scale bar = 200 microns.
