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. 2020 Jul 7;10:980. doi: 10.3389/fonc.2020.00980

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Copyright © 2020 He, Zhang, Liu, Chen, Xie, Peng, Zheng, Xu, Li and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histopathological observation and immunohistochemical verification of TLS. The TLS present in GC tissue (A–D) (blue arrow). MECA-79 and CD21 were used to verify the accuracy of the morphological evaluation of TLS. HEVs (A–C) (red arrow, MECA-79⁺) and venules (A–C) (black arrow, MECA-79⁻) are shown closing around the TLS. CD21-marked FDCs (E,F) (blue arrow, CD21⁺) are observed in the TLS. TLS, tertiary lymphoid structure; GC, gastric cancer; HEVs, high endothelial venules; FDCs, follicular dendritic cells.