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Detection of specific IgA against F. nucleatum in fecal supernatants (FSN) of rats. The western blotting was used to detect the IgA against F. nucleatum at weeks 3 (A), 8 (B), and 12 (C). (D), Ponceau S staining was used as loading control. The quantified proportional signals from reactive bands at 40 and 130 kDa were calculated at weeks 3 (E), 8 (F), and 12 (G). *P < 0.05, **P < 0.01, ***P < 0.001. MS, maternal separation.
