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. 2020 Jul 7;11:1025. doi: 10.3389/fphar.2020.01025

Figure 2.

Figure 2

BA restrains F-actin belt formation and osteoclastic hydroxyapatite resorption triggered by RANKL while decreasing ROS levels in BMMs. (A) Representative images of formed F-actin belts as assessed by immunofluorescent staining with DAPI counterstaining. (B) Mean F-actin belt areas (n = 3). (C) Numbers of nuclei per osteoclast. (D) Representative micrographs of TRAP stained osteoclasts on hydroxyapatite coated plates. (E) Representative images for ROS production in presence or absence of BA in RANKL-treated BMMs. (F, G) Numbers of osteoclasts and areas of bone resorption assessed by the ImageJ software. (H) DCF intensities per cell indicating ROS levels. Data are mean ± SD. **p <0.01, ***p <0.001 vs. RANKL-induced control group (no BA administration). Scale bar = 1,000 μM, BA, Betulinic acid; DAPI, 4′,6‐diamidino‐2‐phenylindole; F-actin, fibrous actin.