Figure 1.

HHT inhibits in vitro allergic reactions. (A) The IgE (DNP-specific)-sensitized RBL2H3 cells (upper) or lung mast cells (lower) were treated without or with various concentrations of HHT for 1 h, followed by treatment with DNP-HSA (100 ng/ml) for 1 h. Immunoblot was performed (upper). (B) The IgE-sensitized RBL2H3 cells were treated without or with HHT (1 μM) for 1 h, followed by DNP-HSA stimulation for 1 h. Immunoblot and immunoprecipitation were performed (right). Immunoprecipitation employing isotype-matched anti-IgG antibody was also performed. (C) The IgE-sensitized RBL2H3 cells were treated without or with HHT at the indicated concentration for 1 h, followed by DNP-HSA stimulation for 1 h. ***p < 0.001.