Figure 2.

HHT inhibits PCA. (A) BALB/C mice were given an intradermal injection of DNP-specific IgE (0.5 μg/kg). The next day, BALB/C mice were given an intravenous injection of HHT (28 μg/kg) for 1 h, followed by an intravenous injection of PBS or DNP-HSA (250 μg/kg) along with 2% (v/v) Evans blue solution for determining the extent of vascular permeability accompanied by PCA. Representative images of each BALB/C mouse of each experimental group are shown. Each experimental group comprises four BALB/C mice. Means ± S.E. of three independent experiments are depicted. ***p < 0.001. (B) Ear tissue lysates were subjected to β-hexosaminidase activity assays. ***p < 0.001. (C) Immunoblot and immunoprecipitation were performed.