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. 2020 Jul 7;11:1032. doi: 10.3389/fphar.2020.01032

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Copyright © 2020 Kim, Jo, Kwon, Kim, Jung and Jeoung

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HHT attenuates clinical symptoms associated with AD. (A) 2, 4-dinitro fluorobenzene (DNFB) induced AD. HHT (28 μg/kg) was intravenously injected at the indicated days of the time line. Clinical scores were determined as described. Each experimental group comprises five BALB/C mice. **p < 0.01; ***p < 0.001. (B) β-hexosaminidase activity assays were performed. ***p < 0.001. (C) The amount of histamine released was determined by using sera of BALB/C mice of each experimental group. **p < 0.01. (D) Same as (B) except that immunoblot and immunoprecipitation were performed. (E) Same as (D) except that lysates of skin mast cells isolated from skin tissue were employed. (F) Skin tissue lysates were subjected to qRT-PCR analysis.