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. 2020 Jul 7;11:1032. doi: 10.3389/fphar.2020.01032

Figure 4.

Figure 4

MiR-183-5p mediates allergic inflammation in vitro. (A) The IgE-sensitized RBL2H3 cells were treated without or with HHT (1 μM) for 1 h, followed by DNP-HSA stimulation for 1 h. MiRNA array analysis was performed. (+) represents increase in expression level of the indicated miRNA in RBL2H3 cells. (-) represents decrease in expression level of the indicated miRNA in RBL2H3 cells. (B) The IgE-sensitized RBL2H3 cells or lung mast cells were treated without or with HHT (1 μM) for 1 h, followed by DNP-HSA stimulation for 1 h. QRT-PCR analysis was performed. **p < 0.01; ***p < 0.001. (C) Twenty-four hours after transfection with the indicated inhibitor, cells were sensitized with IgE for 24 h, followed by DNP-HSA stimulation for 1 h. ***p < 0.001. Each inhibitor was dissolved in DMSO and diluted with PBS. (D) Same as (C) except that immunoblot and immunoprecipitation were performed.