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BTG1 inhibits allergic inflammation in vitro. (A) The indicated construct was transfected. The next day, cells were treated with DNP-specific IgE for 24 h, followed by treatment with DNP-HSA for 1 h. (B) β-hexosaminidase activity assays and qRT-PCR analysis were performed. ***p < 0.001. (C) Twenty-four hours after transfection, cells were treated without or with DNFB for 1 h, followed by immunoblot and qRT-PCR analysis. *p < 0.05; ***p < 0.001.
