Figure 8.

NF-κB mediates allergic reactions. (A) The IgE-sensitized RBL2H3 cells were pretreated without or with BAY 11-0782 (20 μM) for 1 h, followed by treatment with DNP-HSA for 1 h. BAY 11-0782 was dissolved in DMSO and diluted with PBS. (B) QRT-PCR analysis and β-hexosaminidase activity assays were performed. ***p < 0.001. (C) Immunofluorescence staining was performed. (D) HaCaT cells were pretreated without or with BAY 11-0782 (1 μM) for 1 h, followed by stimulation with DNFB for 1 h. Immunoblot and qRT-PCR analysis were performed. **p < 0.01; ***0p < 0.001.