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Limit of detection (LOD) for the targets under study. Each bar represents the positive partitions over the three pooled replicates within the 5 concentration levels (L1: 8,000 cp/μL, L2: 800 cp/μL, L3: 80 cp/μL, L4: 8 cp/μL, and L5: 1 cp/μL) compared to the no template control (NTC). Red line represents the selected threshold: nine positive partitions.
