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Regression analysis for each target sequence evaluated at 95% confidence.
| Gene | Slope | Intercept | R2 |
| invA | 1.0094 | 6.8378 | 0.9996 |
| ttr | 1.0200 | 6.8474 | 0.9990 |
| spaQ | 0.9749 | 6.2832 | 0.9985 |
| hilA | 0.9707 | 6.2528 | 0.9985 |
| siiA* | 1.0063 | 6.3995 | 0.9996 |
*siiA shows good linearity up to fourth Log dilution.
