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. 2020 Jun 11;11:1165. doi: 10.3389/fimmu.2020.01165

Figure 1.

Figure 1

Immunological characterization of the GL261 cell line, and the effects of radiation. The expression of two tumor-associated antigens (ErbB2 and WT1) on GL261 cells and the short-term effects of radiation on the expression of these proteins were confirmed by western blot analysis (A). Fold changes in protein signals are summarized by bar charts (B). The expression of MHC I on GL261 cells and the short-term effects of radiation on this expression were also confirmed by flow cytometry (C). The surface expression and total protein expression of PDL1 on GL261 was estimated by flow cytometry and western blot (D,E). Moreover, the effects of radiation (2, 4, and 6 Gy) on the viability and proliferation of GL261 cells were also clarified by clonogenic assay and MTT assay (F,G). Data is summarized by bar charts as the mean ± standard deviation (SD). All data are represented as the mean of two independent experiments. β-Actin was used as an internal control, and the figure is composed of multiple gel images. Full-length blots are presented in Figure S2. GL261, mouse glioblastoma cell line; H-2Kb, MHC class I expression in mice. *p < 0.05; **p < 0.01; and ***p < 0.001; n.s., no significant difference.