Table 1.
PCR primers used in this study.
Primer | Sequence (5′–3′) | Gene | Amplicon size (bp) | References |
---|---|---|---|---|
Virulence genes | ||||
tdhF | GTAAAGGTCTCTGACTTTTGGAC | tdh | 269 | Bej et al. (1999) |
tdhR | TGGAATAGAACCTTCATCTTCACC | |||
trhF | TTGGCTTCGATATTTTCAGTATCT | trh | 500 | Bej et al. (1999) |
trhR | CATAACAAACATATGCCCATTTCCG | |||
AMR genes | ||||
blaTEMF | ATAAAATTCTTGAAGAC | bla TEM | 1075 | Letchumanan et al. (2015) |
blaTEMR | TTACCAATGCTTAATCA | |||
qnrF | TCTCGCTAAGGCTCGTAGC | qnr | 902 | Poirel et al. (2005) |
qnrR | TTCCTCGTCGAGGTTATTCG | |||
ermBF | AGACACCTCGTCTAACCTTCGCTC | erm(B) | 640 | Raissy et al. (2012) |
ermBR | TCCATGTACTACCATGCCACAGG | |||
strBF | GGCAGCATCAGCCTTATAATTT | strB | 470 | Mala et al. (2016) |
strBR | GTGGATCCGTCATTCATTGTT | |||
sul2F | TGCGGATGAAGTCAGCTCC | sul2 | 623 | Mala et al. (2016) |
sul2R | GGGGGCAGATGTGATCGAC | |||
tetAF | GTAATTCTGAGCACTGTCGC | tet(A) | 957 | Mala et al. (2016) |
tetAR | CTGCCTGGACAACATTGCTT | |||
dfrA1F | CAAGTTTACATCTGACAATGAGAACGTAT | dfrA1 | 277 | Mala et al. (2016) |
dfrA1R | ACCCTTTTGCCAGATTTGGTA | |||
dfrA18R | ACTGCCGTTTTCGATAATGTGG | dfrA18 | 389 | Mala et al. (2016) |
dfrA18F | TGGGTAAGACACTCGTCATGGG | |||
Integrons | ||||
int1F | CCTGCACGGTTCGAATG | intl1 | 497 | Kitiyodom et al. (2010) |
int1R | TCGTTTGTTCGCCCAGC | |||
int2F | GGCAGACAGTTGCAAGACAA | intl2 | 247 | Kitiyodom et al. (2010) |
int2R | AAGCGATTTTCTGCGTGTTT | |||
int3F | CCGGTTCAGTCTTTCCTCAA | intl3 | 155 | Kitiyodom et al. (2010) |
int3R | GAGGCGTGTACTTGCCTCAT | |||
ICEs | ||||
intSXTF | GCTGGATAGGTTAAGGGCGG | int SXT | 592 | Kitiyodom et al. (2010) |
intSXTR | CTCTATGGGCACTGTCCACATTG |