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| Reagent | Initial concentration | Volume (µL) | Final concentration |
| Buffer | – | 20 | – |
| PLP | 500 µM | 8 | 40 µM |
| Compound | * | 30 | * |
| Total: | 58 µL |
*The initial concentration of the testing compound must be 3.33 times higher than the desired final concentration (adjusted into 30 µL of 100 µL). This is the ratio between the initial and final concentration to reach the standardized volumes (30 µL of testing compound in a total of 100 µL of reaction).
