Figure 2.

The Expression of Ormdl Genes in the Islets of Leptin-Deficient Obese (ob/ob) Mice

Quantitative PCR analyses of Ormdl1, Ormdl2, and Ormdl3 mRNA expression in primary islets of 10-week-old male (n = 8 per group) (A–C) and female (D–F) lean (n = 9) and obese (ob/ob) (n = 8) mice. (G) Representative western blot image (of two independent experiments performed) reflecting Ormdl protein expression in the islets of lean and ob/ob male mice determined by western blot using the validated TPF-Ormdl antibody. All data are expressed as ΔCT (versus β-actin) and represented as mean ± SEM (∗∗∗p < 0.001). ns: non-significant.