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. 2020 Jul 5;31:106001. doi: 10.1016/j.dib.2020.106001
Subject Ophthalmology
Specific subject area Lipids
Type of data Chart
Graph
Figure
Chromatograms
Spectra
How data were acquired Liquid Chromatography Q-Exactive Orbitrap Mass Spectrometry
Data format Raw
analysed
Filtered
Parameters for data collection Optic Nerves, Optogenetics, Light stimulation
Description of data collection Optic nerves from transgenic channelrhodopsin mice (Thy1-ChR2-EYFP mice) and C57BL/6 J mice were collected. Thy1-ChR2-EYFP mice and controls were divided in four groups each (n = 6) – 1) no crush and no stimulation, 2) no crush and stimulation, 3) crush and no stimulation, and 4) crush and stimulation. Optic nerves were collected, and lipid extraction was performed with the Bligh and Dyer method. The samples were analysed untargeted for lipids with Liquid Chromatography Q-Exactive Orbitrap Mass Spectrometry.
Data source location Bascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL 33,136, USA
Data accessibility Study ID ST001381 at Metabolomics Workbench Repository: https://www.metabolomicsworkbench.org/data/DRCCMetadata.php?Mode=Study&StudyID=ST001381&StudyType=MS&ResultType=5