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. 2020 Jun 29;23(7):101320. doi: 10.1016/j.isci.2020.101320

Figure 1.

Figure 1

KLF3 Expression Is Increased during Epidermal Differentiation

(A) Heatmap of replicate RPKM normalized, Log2 transformed RNA-sequencing data showing the relative expression of KLF3 and a panel of differentiation genes in keratinocytes cultured in growth media (GM) versus differentiation media (DM, day 3 and day 6 differentiation). Red color denotes increased expression and blue color denotes decreased expression.

(B) RT-qPCR analysis of KLF3 mRNA levels in a time course of keratinocyte differentiation done in two-dimensions, with day 0 representing undifferentiated keratinocytes and days 1–5 representing progressively differentiated keratinocytes. n = 3. Data are graphed as the mean ± SD. Statistics: t test, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

(C) Western blot of KLF3 protein levels in a time course of keratinocyte differentiation with B-Tubulin as a loading control. Representative image is shown, n = 3.

(D) Immunofluorescent staining of KLF3 (green) and KRT10 (red, a marker of the differentiated layers) in human skin biopsy. Merged image includes Hoechst staining of nuclei. n = 3. Scale bar: 20 μm.

(E) Quantitation of the signal intensity of KLF3 staining in the basal, spinous, and granular layer from the images acquired in (D). Data are graphed as the mean ± SD. Statistics: t test.