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. 2020 Jul 13;22:75. doi: 10.1186/s13058-020-01312-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — a Scatter plot of proteins with significantly higher intensities in PGRMC1-HA samples compared to PGRMC1-GFP samples identified by mass spectrometry. The most significant proteins exhibit very high value for Student’s t test difference HA_GFP and –log Student’s t test p value HA_GFP and are found in the upper right corner. Highlighted are proteins with important functions in steroid synthesis. b Detection of co-immunoprecipitated proteins CYP51A1, Stearoyl-CoA desaturase (SCD1), and FDFT1 by western blot. c Verification of the interactions via proximity ligation assay. Quantification of dots per cell. d Visualization via immunofluorescence microscopy. e Quantification of protein expression of CYP51, SCD1, and FDFT1 in MCF7/PGRMC1 cells and MDA-MB-231/PGRMC1 cells compared to their respective empty vector control by western blot. *p ≤ 0.05, ***p ≤ 0.001. f Detection of cholesterol and its precursor lathosterol in PGRMC1 overexpressing cells compared to the empty vector control cells with mass spectrometry *p ≤ 0.05, ***p ≤ 0.001 (Student’s t test, n = 3)