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. 2020 Jul 13;22:75. doi: 10.1186/s13058-020-01312-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — a Protein phosphorylation of EGFR P-Tyr1068, Akt P-Ser473, Akt P-Thr308, MEK1/2 P-Ser217/Ser221, Erk1/2 P-Thr202/Tyr204, and S6 P-Ser240/Ser244 analyzed by RPPA. NFI (blank-corrected mean fluorescence intensity) ratio of phospho-protein/total protein was calculated, normalized to MCF7/EVC, and ratio in MCF7/EVC cells was set to 1. Up-/downregulation of protein phosphorylation in MCF7/PGRMC1 cells was calculated. *p ≤ 0.05, **p ≤ 0.01 (Student’s t test, n = 3). b Protein phosphorylation of EGFR P-Tyr1068, Akt P-Ser473, MEK1/2 P-Ser217/Ser221, and Erk1/2 P-Thr202/Tyr204 verified by western blot analysis. Cells were treated with EGF (10 ng/mL) for 10 min/37 °C. Representative blot of 3 independent analyses. Total protein expression of EGFR, Akt, MEK1/2, and Erk1/2 verified by western blot analysis. Representative blot of 3 independent analyses shown. c PGRMC1 mediates phosphorylation of EGFR and its downstream targets and upregulates E2 levels, ERα expression, and ERα-target genes. EGFR phosphorylation activates the MAPK signaling cascade (including MEK1/2-, ERK1/2-, and S6-phosphorylation) and PI3K signaling cascade (including Akt- and S6-phosphorylation). Phosphorylation of S6 induces transcription of genes, involved in the regulation of cell cycle progression, cell proliferation, and glucose homeostasis. ERα translocates into the nucleus upon ligand-dependent or ligand-independent activation and acts as a transcription factor to transcribe genes involved in tumor progression. d Overview of the influence of PGRMC1 in cholesterol and lipid metabolism. e MCF7/EVC and MCF7/PGRMC1 cells were treated with 100 μM, 50 μM, 25 μM, 12.5 μM, 6.25 μM, and 3.175 μM simvastatin and respective DMSO control. MDA-MB-231/EVC and MDA-MB-231/PGRMC1 cells were treated with 20 μM, 10 μM, 5 μM, 2.5 μM, 1.25 μm, and 0.625 μM simvastatin and respective DMSO control. Viability was analyzed by MTT assay at t = 24 h, t = 48 h, t = 72 h and 37 °C. Depicted are results after 48 h of treatment. Viability is normalized on the DMSO control. p values were adjusted using the Bonferroni correction (n_doses = 6; n_replicates = 9)