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. 2020 Jul 13;11:280. doi: 10.1186/s13287-020-01806-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Differentiation evaluation of CM-Dil-labeled hUC-MSCs. The target protein color was green, the DAPI with Dil-hUC color was magenta representing the living cells, and the superposition color of green and magenta was white which means differentiated cells (arrow). CK7 was expressed in the cytoplasm of epithelial cells, CD31 was expressed in the cytoplasm of vascular endothelial cells, and ERα was expressed in the nucleus and cytoplasm. From the 2nd week of treatment, hUC-MSCs began to differentiate into epithelial cells, vascular endothelial cells, and a small amount of ER cells