Figure 1.

Experimental timeline for contralateral tumor self-homing model (n=5) (A). On Day 0 after cell injection, 4T1-RLuc cells only showed signal after administration with h-coelenterazine and on Day 1, 4T1BR5-FLuc cells only showed signal after administration of D-Luciferin. By day 7, 4T1BR5-FLuc cells did not appear to migrate as FLuc signal was not detected in the contralateral MFP but 4T1-RLuc cells could be detected in the contralateral MFP tumor (B). RLuc signal on day 7 was significantly higher in the contralateral MFP compared to the ipsilateral MFP on day 8 (C-D). The presence of both 4T1-RLuc and 4T1BR5-FLuc cells in the left MFP was confirmed histologically and compared to a control MFP tumor composed of only FLuc expressing cells (scale bars=200 microns) (E).