Figure 2.

The K7R mutant of Rab22a-NeoF1 lacks its capacities of enhancing migration, invasion and metastasis in vitro and in vivo. (A, B) Quantification analyses of migration and invasion assays (lower panel) using U2OS cells or U2OS/MTX300 cells stably expressing Vector, Rab22a-NeoF1 (WT) or its K7R mutant (K7R), as indicated. The expression level of WT and K7R Rab22a-NeoF1 in U2OS cells and U2OS/MTX300 cells were shown in the upper panel. (C-F) The orthotopic osteosarcoma metastasis model in vivo using the U2OS/MTX300-Luc cells stably expressing Vector, Rab22a-NeoF1 (WT) or its K7R mutant (K7R), as indicated. Representative images of mice (C). H&E staining of the lungs from representative tumor-bearing nude mice (D). Quantification analyses of Log₁₀(Average Radiance) of lung metastasis. (E) Quantification analyses of lung nodules (F). Quantification analyses of wet lung weight (G) from the nude mice used in C.