sEH inhibition alters epoxide, diol and EPHX levels. (A) Levels of 19,20-EDP and 19,20-DHDP in retinae from PKD rats treated with vehicle or sEH-I Sar5399 (30 mg/kg) for four weeks; n = 5; *P < 0.05 (two-way ANOVA with Sidak's multiple comparisons test). (B) Levels of 19,20-EDP and 19,20-DHDP in serum from vehicle- and sEH-I-treated PKD rats; n = 6; **P < 0.01 (two-way ANOVA with Sidak's multiple comparisons test). (C) Effect of sEH inhibition on levels of arachidonic acid-derived EET and DHET levels in retinae from PKD rats; n = 5; **P < 0.01 (two-way ANOVA with Sidak's multiple comparisons test). (D) Relative mRNA expression of epoxide hydrolase isozymes in SD rats, as well as vehicle- and sEH-I treated PKD. mEH = microsomal epoxide hydrolase (or EPHX1); sEH, (EPHX2); EPHX3 and EPHX4; n = 3; *P < 0.05, ***P < 0.001 (two-way ANOVA with Tukey's multiple comparisons test).