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. 2020 Jun 19;10(17):7775–7786. doi: 10.7150/thno.46903

Figure 2.

Figure 2

GLRX5 genetic silencing increases ferroptosis and lipid peroxidation. (A-C) Cell death and protein expression in HN4 and HN4R cells stably transduced with shRNA targeting GLRX5 or vector control (vtr). The cells were subjected to 0.5 mM SAS for 48 h. Scale bar, 50 μm. ** P < 0.01 relative to a non-vector control (ctr) or vtr. (D-F) Cellular ROS, lipid ROS (BODIPY), and glutathione (GSH) contents in HN4R cell with or without GLRX5 gene silencing, which were subjected to 1 mM SAS treatment for 8 h. The error bars represent standard errors from three replicates. ** P < 0.01 relative to ctr or vtr. NS indicates statistically not significant.