Figure 4.

Resistant GLRX5 cDNA transduction rescues ferroptosis and free iron increases. (A-C) Immunoblots, cell death, and lipid ROS in HN4R cells stably transduced with shRNA targeting a vector or GLRX5 and resistant GLRX5 cDNA (GLRX5res). The cells were exposed to 0.5 mM SAS for 48 h (PI+ cells) or 1 mM SAS 8 h (BODIPY). (D-F) LIP, Fe²⁺, and mitochondrial iron assays in HN4R cells stably transduced with a vector or shGLRX5 and GLRX5res, which were exposed to 1 mM SAS treatment for 8 h. The error bars represent standard errors from three replicates. Scale bar, 50 μm. ** P < 0.01 between the different groups treated with SAS.