Figure 3.

CD133 pattern in LS1034 cells in vitro does not determine engraftment and in vivo behavior; CD133/CD44 cell surface profiles and distributions in LS1034 xenografts are consistent, with CD44 been newly expressed on a CD133⁺ subpopulation. (A) Representative flow cytometric dot blot diagrams of CD133-PE and CD44-APC surface pattern in LS1034 cells before sorting (cf. Figure 1) and CD133 histograms of CD133^- (1) and CD133⁺ (2) subfractions after FACSorting before s.c. injection; bottom dot blots document representative CD133/CD44 pattern in cell suspensions prepared from xenografts originated from the respective injected subfractions; (B) Engraftment rates of LS1034 cell subfractions separated by FACS according to their in vitro CD133 surface expression; control = “run-through-sorter” (cf. Figure 2). (C) Tumor control as function of time after s.c. injection of 100 CD133^- or CD133⁺ LS1034 cells (according to B). (D) Normalized volume growth kinetics of xenografts derived from 500 CD133^- or CD133⁺ LS1034 cells; (E) Distribution and proportion, respectively, of CD133⁺, CD44⁺, and CD133/CD44 single or double negative and positive LS1034 cells (+SD) in xenografts originated from either CD133⁺ or CD133^- FACSorted in vitro cells.