Figure 4.

CD133/CD44 pattern in LS1034 primary xenografts correlates with secondary engraftment. (A) Representative flow cytometric dot blot diagrams of CD133-PE and CD44-APC surface pattern in suspensions of LS1034 xenograft cells before (cf. Figure 3) and after FACSorting and following secondary engraftment; (B) Engraftment rates of LS1034 xenograft cell populations separated by FACS according to their in vivo CD133/CD44 surface expression; control = “run-through-sorter” (cf. Figure 2). (C) Tumor control as function of time after s.c. injection of 2,500 FACSorted LS1034 cells originated from xenografts; *** p<0.001; (D) Normalized volume growth kinetics of secondary xenografts derived from 10,000 CD133⁺/CD44^- or CD133⁺/CD44⁺ LS1034 in vivo cells; (E) Distribution and proportion, respectively, of CD133⁺, CD44⁺ and CD133/CD44 single or double negative and positive LS1034 cells (+SD) in secondary xenografts originated from CD133⁺/CD44^- or CD133⁺/CD44⁺ cell populations isolated from primary LS1034 xenografts.