Figure 5.

CD44⁺ tumor cells are located in well-oxygenized but not in a proposed hypoxic cancer stem cell niche in LS1034 xenografts (see also Figure S4 A, B). Median frozen section (10 µm) of an LS1034 xenografts co-stained for CD44, CD31 (endothelial cells), pimonidazole accumulation (hypoxia), and DAPI (nuclei) and imaged with a magnification of 200x; whole tumor section (stitched from >1,000 single images - top) and a selected region at higher magnification are displayed as four-channel overlays, while single channel images of the respective region are documented on the bottom.