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. 2020 Jun 12;10(17):7492–7509. doi: 10.7150/thno.44309

Figure 3.

Figure 3

Inhibition of miR-181a-5p decreases osteogenic property of OPLL cells. Alkaline phosphatase staining (A) or alizarin red staining (B) were used to analysis osteogenic properties of miR-181a-5p inhibition (181a-5p-Inh), miR-181a-3p inhibition (181a-3p-Inh) and miR-10a-3p inhibition (10a-3p-Inh) in OPLL cells after osteo-induction for 21 days. The colorimetric quantification is shown in the right panels, respectively. NC group represents transfecting scramble control miRNA mimics. Ossification-related genes are assessed by real-time PCR (C) and Western Blot (D) after osteo-induction for 21 days under the same conditions respectively. All PCR experiments were repeated three times individually, and GAPDH level were detected and served as internal reference. All data were presented as the mean ± SD. *P < 0.05, **P < 0.01.