Fig. 3.

Validation of proteomic analysis. To validate the proteomic analysis, western blotting was performed and analyzed the expression of proteins, nestin, heme oxygenase-1 (HO-1), and clathrin light chain B after sham or ICH, as detailed in methods (a). Consistent with the proteomic studies, the densitometry analysis of the western blotting data demonstrated significant upregulation of nestin (b) as well as heme oxygenase-1 (HO-1) (c) and significant downregulation of clathrin light chain B (d) after ICH in comparison to sham. β-actin was used as a loading control (** p < 0.01, * p < 0.05 vs. sham). n = 4 animals/group