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Increase in selectivity of Topi modification in nuclear extract using M13mpl9 phage DNA. Radiolabeled M13mpl9 phage DNA (1, 2) or 34-mer DNA duplex (3, 4) were incubated with nuclear extract (NE) proteins (1, 3) or with Top1 (2, 4). Modification products were analyzed by SDS-PAGE.
