Table 1.

Summary of isolates, iOTUs, singletons and coverage per depth

	99% (non-rarefied)			99% (rarefied)
	Photic	Mesopelagic	Bathypelagic	Photic	Mesopelagic	Bathypelagic
Number of isolates	817	362	382	346	362	368
Number of iOTUs	100	57	59	61	57	59
Number of Singletons	39	25	20	18	25	20
Good’s coverage	61%	56.1%	66.1%	70.5%	56.1%	66.1%

Results derived from isolates clustering at 99% sequence similarity to construct the non-rarefied and rarefied iOTU-abundance table (sampled down to the layer with the lowest number of isolates, i.e. mesopelagic with 362 isolates). Singletons: iOTUs appearing only once