Table 2.
Origin of the novel Mesonia strains ISS653 and ISS1889 and phenotypic and genetic main differences.
| ISS653 | ISS1889 | |
|---|---|---|
| Station | ST 151 | ST 102 |
| Ocean | North Atlantic Ocean | South Pacific Ocean |
| Depth (m) | 5 | 475.6 |
| In situ Temperature (°C) | 17.3 | 9.2 |
| Physiology differences: | ||
| Maximum temperature for growth (°C) | 37 | 30 |
| Tween-80 hydrolysis | weak | – |
| Phenylacetate assimilation (API20NE) | + | – |
| Acid from (API50CH/E): | ||
| arbutin | – | weak |
| 2-ketoglutarate | – | weak |
| Cellular fatty acids: | ||
| iso-C15:0 2OH | 11.2 | 14.5 |
| iso-C17:0 3OH | 9.2 | 14.6 |
| iso-C17:1 ω9c | 5.2 | 10.5 |
| C15:1 ω5c | 3.6 | Traces (< 1%) |
| C18:1 ωc | 3.9 | – |
| Genomic differences: | ||
| Genome size (bp) | 4,275,762 | 4,283,636 |
| G + C content (mol%) | 34.9 | 34.9 |
| RNAs | 45 | 45 |
| Protein codifying sequences: | 4030 | 4015 |
| Chaperones | GroEL, GroES, ClpB | – |
| Cobalt-zinc-cadmium resistance | CusA, CzcA, CzcD | – |
| Mercury resistance | – | MerA, MerT |
Differences extracted from a total of 158 tests (Supplementary Methods in Additional file 1). Within protein codifying sequences we included a list of some interesting proteins that were unique for one of the strains