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. 2020 Jul 13;11:58. doi: 10.1186/s13229-020-00360-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Sections of (a) 30-day, (b) 45-day, and (c) 65-day-old cerebral organoid grown from wild-type human iPSCs. a Immunostaining for progenitor marker PAX6 (red) and deep layer neuronal marker CTIP2 (green). b Immunostaining for intermediate progenitor marker TBR2 (green), deep later neuronal marker TBR1 (red), and deep layer neuronal marker CTIP2 (white). c Immunostaining for progenitor marker PAX6 (yellow), deep layer neuronal marker CTIP2 (red), and upper layer neuronal marker SATB2 (green). Progenitors are located interiorly, near the central lumen whereas differentiated neurons are located towards the outer edge of the organoid and multiple time points show that neuronal layers are progressively established, resembling in vivo embryonic cortex. L, lumen, O, outer edge. Scale bar, 100 μm