Fig. 1.

Doxorubicin or H₂O₂ induces hUC-MSC senescence. 2 × 10⁵ hUC-MSCs (p5-p7) were seeded in 6-well plates and treated with Dox (10⁻⁸ M) for 24 h or H₂O₂(300 nM) for 3 h, then washed with PBS and grown for another 2–4 days. After that, the cells were subjected to various analyses. a β-Gal staining was conducted at day 2 or day 4 in Dox- or H₂O₂-induced hUC-MSCs (scale bar = 100 μm). The quantification of β-Gal staining is shown on the right. b Two thousand hUC-MSCs were seeded in 6-well plates and treated with Dox (10⁻⁸ M) for 24 h or H₂O₂(300 nM) for 3 h, then washed with PBS and grown for another 7 days. c qRT-RCR analysis of cell cycle-related genes in Dox- or H₂O₂-induced hUC-MSCs; cells were collected at day 2 or day 4. d qRT-PCR analysis of senescence-associated secretory phenotype (SASP) genes in Dox- or H₂O₂-induced hUC-MSCs; cells were collected at day 2 or day 4. Data are presented as the mean ± SEM. **p < 0.01;***p < 0.001 by Student t test