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. 2020 Jul 13;11(7):531. doi: 10.1038/s41419-020-2735-2

Fig. 4. PDCD4 is a direct target gene of miR-21.

Fig. 4

a The binding site of PDCD4 in miR-21 was predicted via miRDB. b The indicated WT- or MUT-PDCD4 3′-UTR luciferase vectors and miR-21 mimic/NC were co-transfected into 293T cells. Transfection with the miR-21 mimic significantly reduced the luciferase activity of the PDCD4-WT reporter. c MiR-21 expression was knocked down by transfection with the miR-21 inhibitor and overexpressed by transfection with the miR-21 mimic. d Relative protein levels of PDCD4 in HeLa and SiHa cells transfected with the miR-21 inhibitor, miR-21 inhibitor NC, miR-21 mimic or miR-21 mimic NC. The data are shown as the means ± SDs (n = 3). **P < 0.01, ***P < 0.001.