Fig. 5. The miR-21 mimic reverses the apoptosis-promoting function of GAS5 through the PDCD4 pathway.

a Flow cytometric analysis showed that introduction of the miR-21 mimic attenuated the apoptosis-promoting effect of GAS5 in SiHa cells. The lower right quadrant of each apoptosis diagram is the early apoptotic cells and the upper right quadrant is the late apoptotic cells. The percentages of apoptotic cells were the sum of the early and late apoptotic cells and were compared among the groups. b Si-PDCD4 was transfected into HeLa and SiHa cells to achieve PDCD4 knockdown. The transfection efficiency was verified using RT-PCR and western blotting. c Flow cytometric analysis showed that si-PDCD4 inhibited the apoptosis of SiHa cells. d Western blot analysis showed that knockdown of PDCD4 reduced the levels of cleaved PARPγ and cleaved caspase‐3 after treatment with cisplatin for 48 h in HeLa and SiHa cells. e Western blot analysis revealed that introduction of the miR-21 mimic attenuated the effect of GAS5 on PDCD4, cleaved PARPγ and cleaved caspase‐3 levels in CC cells. The data are from triplicate experiments and are presented as the means ± SDs. *P < 0.05 and **P < 0.01.