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. 2020 Jul 13;11(7):530. doi: 10.1038/s41419-020-2719-2

Fig. 3. YAP and β-catenin promote docetaxel and vinorelbine resistance in TNBC cells.

Fig. 3

a, b The CCK-8 assay showed that MDA-MB-231/DR and MDA-MB-231/VR cells were more resistant to docetaxel and vinorelbine, respectively, than MDA-MB-231 cells. c The protein expression of YAP and β-catenin in drug-resistant and drug-sensitive cells was detected by western blot assays. d CCK-8 assays for MDA-MB-231 cells transfected with control or pCMV-Flag-YAP-5SA plasmid cultured with docetaxel at different concentrations for 48 h. Control group vs YAP group (1P = 0.013, 2P = 0.016, 3P = 0.006, 4P = 0.005). The rest of the CCK-8 assays were similar to those described above. e siRNA-YAP was used for YAP knockdown. Control group vs si-YAP group (1P = 0.003, 2P < 0.001, 3P = 0.002, 4P = 0.008). f pcDNA3.0-β-catenin was used for β-catenin overexpression. Control group vs β-catenin group (1P = 0.008, 2P < 0.001, 3P = 0.002, 4P = 0.002). g siRNA-β-catenin was used for β-catenin knockdown. Control group vs si-β-catenin group (1P = 0.004, 2P < 0.001, 3P = 0.002, 4P = 0.006, 5P = 0.002). h pCMV-Flag-YAP-5SA was used for activated YAP. Control group vs YAP group (1P = 0.016, 2P = 0.012, 3P = 0.009, 4P = 0.009). i siRNA-YAP was used for YAP knockdown. Control group vs si-YAP group (1P = 0.034, 2P = 0.009, 3P = 0.008, 4P = 0.009). j pcDNA3.0-β-catenin was used for β-catenin overexpression. Control group vs β-catenin group (1P = 0.006, 2P = 0.003, 3P = 0.003, 4P = 0.014). k siRNA-β-catenin was used for β-catenin knockdown. Control group vs si-β-catenin group (1P = 0.031, 2P = 0.019, 3P = 0.002, 4P = 0.003). The data are presented as the mean ± SD of experiments performed in triplicate. Statistical significance was determined using Student’s t test analysis.