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. 2020 Jul 13;11(7):530. doi: 10.1038/s41419-020-2719-2

Fig. 6. Aspirin inhibited YAP and β-catenin by upregulating β-TrCP.

Fig. 6

a The cells were cultured with different concentrations of aspirin (0, 2.5, 5 and 10 mM) for 48 h. Then, a western blot assay was employed to detect the protein expression of YAP and β-catenin. b β-TrCP protein was detected by western blot after treatment with different concentrations of aspirin (0, 2.5 and 5 mM). c The cells were divided into different groups: blank group (cultured with normal medium), NC (negative control) group (transfected with negative control siRNA), and si-β-TrCP group (transfected with siRNA for β-TrCP). The protein expression levels of β-TrCP, YAP and β-catenin were examined by western blot assay in the above cells. d The cells were divided into different groups: control group (cultured with DMSO and transfected with negative control siRNA), aspirin group (cultured with 5 mM aspirin), and aspirin + si-β-TrCP group (cultured with 5 mM aspirin and transfected with siRNA for β-TrCP). The protein expression levels of YAP and β-catenin were examined by western blot assay in the above cells and performed in in triplicate.