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. 2020 Jul 13;10:11507. doi: 10.1038/s41598-020-68109-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Light and electron microscopy (CSEM and TEM) results. (A, B) 3D reconstruction-derived surface roughness (R_a) in ileum (A) and colon (B) (bar represents median, whiskers IQR). CSEM images of small intestine of a septic (C) and a control (D) mouse. Intercellular distances at Desmosome (E), AJ (F) and TJ level (G) (bar represents median, whiskers IQR). Bottom row: light microscopy of a control (H) and a septic (I) mouse (H&E staining, magnification × 400, scale bars represent 20 µm). Septic animals showed significantly increased intercellular distances at the basal levels of the IECs (J).