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. 2020 Jul 13;11(7):526. doi: 10.1038/s41419-020-02745-x

Fig. 1. Mir-20a-5p regulates apoptosis, autophagy, and angiogenic function of EPCs induced by high glucose in vitro.

Fig. 1

EPC dysfunction was induced by high glucose (30 mM) treatment for 24 h. Mannitol was used as an osmolar control treatment. EPCs were transfected with mir-20a-5p or negative control (NC) inhibitor, or mir-20a-5p or NC mimic. a RT-qPCR analysis of mir-20a-5p expression. b Flow-cytometric analysis of apoptosis using annexin V/propidium iodide (PI). Annexin V+/PI+ or Annexin V+/PI− (quadrants 2 and 3) cells were defined as apoptotic cells. c Protein levels of BCL-2, BAX, and cleaved-CASP3 as detected by western blotting. d Representative images showing LC3 staining in different groups of EPCs infected with GFP-RFP-LC3 adenovirus for 24 h. Scale bar: 20 μm. e Western blot analysis of LC3B-II/LC3B-I and SQSTM1/p62 levels. f The angiogenic capability of EPCs was determined by a tube formation assay. Tube length was normalized to that in the control group. Scale: 200 μm. g Protein levels of VEGF as detected by western blotting. Inh inhibito, Mic mimic, *P < 0.05, **P < 0.01, n = 3.