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. 2020 Jul 13;11:3492. doi: 10.1038/s41467-020-17270-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Experimental design for vCA1 projection-specific Ca²⁺ imaging. Cre-dependent GCaMP6f was virally expressed in vCA1 and CAV2-Cre injected in either the BA (top) or LHA (bottom) in order to selectively express GCaMP6f in vCA1-BA or vCA1-LHA projecting neurons. The main effects of this experiment were consistent across all mice within each group. b vCA1-BA and vCA1-LHA mice exhibited similar freezing across contextual fear conditioning Ca²⁺ imaging days (repeated-measures ANOVA; % freezing*group interaction, F_(1,6) = 0.46, p = 0.48, N_BA = 4, N_LHA = 4). c The vCA1-LHA (but not vCA1-BA) projection has a significant decrease in Ca²⁺ event rate during A2 retrieval (BA N_cells = 25, LHA N_cells = 68|Mann–Whitney between groups with Bonferroni alpha correction; A2–A1 U = 681.00, p = 0.14, B–A1 U = 798.50, p = 0.66|Friedman across 3 days (within-group) with post-hoc wilcoxon sign rank A1:A2 and B:A1 (with Bonferroni alpha correction); BA: Friedman X²(2) = 2.06, p = 0.36, A1:A2 Z = −1.26, p = 0.36; LHA: Friedman X²(2) = 9.93, p = 0.0070, A1:A2 Z = −3.34, ##p = 0.0008, B:A1 Z = −0.45, p = 0.65). d Example Ca²⁺ traces from a vCA1-BA (left) and vCA1-LHA (right) FOV during A1 context encoding showing shock responsive neurons in red and Ca²⁺ transients during the shock marked with an asterisk. e The vCA1-BA projection has significantly more shock cells than the vCA1-LHA projection (Chi-squared test of proportions X²(1) = 5.10, *p = 0.0240, N_BA = 25, N_LHA = 68). Error bars, ±s.e.m. Statistical tests comparing distributions were two-sided. Source data are provided as a Source Data file.