Figure 1.

PTX induces quiescent cancer cells with drug resistant capability and stem-like features. Cells were treated with PTX for 7 days (Quiescent), then partially released into medium with half of the initial concentration of PTX and cultured for 3 days (Reac). (A) Cell cycle were analyzed by FACS. (B) Cell proliferation was detected by EdU incorporation assay. Data are shown as mean ± SD of three independent experiments, * P<0.05. (C)The expression of Cyclin D1 were detected by Western blot, GAPDH was used as loading control. (D) Stemness related genes expression were examined by real-time PCR. (E) CD34 and CD133 of cancer cells were detected by flow cytometry.